VANGUARD

Antibody generation and evaluation play an important role in new drug development and biomedicine. Traditional antibody generation usually requires experimental screening to obtain effective antibody sequences, which often requires a lot of time and resources. Experimental screening may not identify all potential high-affinity or high-specificity antibodies, resulting in the risk of missed screening.

AbSeqVAE models the complex distribution of antibody sequences through latent space and obtains the ability to generate new samples. We demonstrated that the improved variational autoencoder model exhibits strong sequence diversity and consistency in antibody generation.

VANGUARD provides a powerful tool for rapid screening of diverse antibodies. The generated antibody sequences need to undergo multiple key evaluations, including solubility, OASis Percentile, OASis Identity, humanization, extinction coefficient, hydrophobicity, molecular weight, isoelectric point, etc., to find the sequence that best meets the needs of practical applications.

In addition, CDRH3 (heavy chain complementary determining region 3) directly affects the binding specificity and affinity of antibodies. Its amino acid sequence varies greatly and is one of the main determinants of antibody-antigen binding. Based on this importance, we performed the same independent evaluation for CDRH3 to help us screen antibody sequences with greater potential.